Enhanced hybridoma formation, clonability and Ab secretion

The isolation and production of human monoclonal antibodies is becoming an increasingly important pursuit. As such, optimization of monoclonal antibody technologies is important, as this is becoming the new rate-limiting step for discovery and development of new pharmaceuticals. It was shown that nanoparticle doping of RF irradiated water (Neowater) stabilizes the RF ordering effect and the water maintain its characteristic properties for extended periods of time, enabling  studied in systems that required prolonged periods for analysis. Since the formation of hybridoma cells involves the formation of a new membrane, a process that is affected by the surrounding aqueous  environment. Experiment at Do-Coop Technologies facility indicate an overall enhancement of hybridoma yield, viability, clonability and secretion and a lot to lot Reproducibility of hybridoma production. Furthermore, we have demonstrated that immortal cells proliferate faster whereas primary human fibroblasts  Proliferate slower in Neowater.